KRT8 anticorps (AA 343-357)

N° du produit ABIN3024645

L’anticorps Souris Monoclonal anti-KRT8 a été validé pour IF, FACS et IHC (p). Il convient pour détecter KRT8 dans des échantillons de Humain.

Aperçu rapide pour KRT8 anticorps (AA 343-357) (ABIN3024645)

Antigène: KRT8 (Keratin 8 (KRT8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT8 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM192

Détail du produit anti-KRT8 anticorps

Épitope: AA 343-357

Aucune reactivité croisée: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: A keratin preparation from a human carcinoma was used as the immunogen for this Cytokeratin 8 antibody. The epitope of this Ab is located between amino acids 343-357.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the Cytokeratin 8 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytokeratin 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur KRT8

Antigène: KRT8 (Keratin 8 (KRT8))

Autre désignation: Cytokeratin 8

Sujet: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.